1. Description

EmerTher® Magnetic FFPE Tissue RNA Extraction Kit is used for extracting RNA from formaldehyde fixed-paraffin embedded (FFPE) tissue.

The Kit contains superparamagnetic nanoparticles which are bound to nucleic acids and an efficient extraction system. The magnetic beads are coated through a unique process, enabling strong binding with nucleic acids and easy elution.

The experimental procedure is simple and efficient:

1) following pretreatment, add sample to a lysis-binding buffer to enable cell lysis, RNA release and RNA binding to magnetic beads in one step.

2) apply magnetic force enabling easy wash of the beads with buffers.

3) elute RNA from the beads using an elution solution. The extraction procedure is fully compatible with automation.

Purified RNA can be directly used in a variety of downstream experiments, including PCR, gene sequencing, etc.

2. Features

Eliminate the need for xylene, a toxic solvent commonly used for dissolving paraffin.

Eliminate the use of toxic chloroform and phenol.

Convenient: No tissue grinding is necessary

Effectively remove cross-linking between nucleic acids and proteins formed during formalin fixation, preventing inhibition of reverse transcription of the extracted RNA due to cross-linkage and increasing the sensitivity and consistency of subsequent tests.

Retain large RNA pieces, facilitating subsequent testing

Automation: compatible with a variety of automatic magnetic bead processors; pre-packed plates are available.