Axela Bio, a CRO specializing in discovery biology and Translational Sciences.
Lumit™ Immunoassay
Lumit™ Immunoassays are based on NanoLuc® Binary Technology.
Antibodies are chemically labeled with small and large subunits of Luciferase, known as SmBiT and LgBiT, respectively. In the presence of an analyte, the two antibodies come into close proximity, allowing SmBiT and LgBiT to form an active enzyme and generate a bright luminescence signal.
Advantages over conventional immunoassays include:
- Simple add-mix-read protocol with no washing steps
- Direct analyte measurement in the cell culture or medium removed from the cells
- Sensitive luminescence detection with wide dynamic range
- Simultaneously measure cell growth and protein expression/changes
We provide customized assays for many cytokines, intracellular proteins, or signaling molecules.
DELFIA Immunoassay
- DELFIA is a heterogeneous, time-resolved fluorescence (TRF) assay that detects reagents labeled with lanthanide chelates (Eu, Tb, and Sm).
- This assay offers excellent flexibility for a range of applications when the desired assay must be highly sensitive, specific, reliable, and reproducible. It is ideal for complex sample matrices and is particularly well suited for TRF, kinase, and immunodetection assays, and discovery work.
- Our DELFIA has a wide dynamic range and is ideal for post-screening applications (i.e., PK, ADA, biomarker, and cell-based assay support).
ProQuantum Immunoassay
- Utilizing proximity-based amplification technology, ProQuantum assays offer the analyte specificity of antibody–antigen binding with signal detection and amplification capabilities of qPCR to achieve high sensitivity (~ 0.01 pg/mL).
- The ProQuantum immunoassay features greater sensitivity and dynamic range than traditional methods while using only 2 μL of sample.
- This assay is particularly useful when handling very limited volumes of precious sample from blood or tumor extracts.
- While it is challenging to detect low levels of protein with limited sample volumes, ProQuantum’s technology solves this by amplifying the signal beyond the capabilities of other available methods.
Immuno-PCR (iPCR, Pico-LISA)
- An ultra-sensitive method for detecting ultra-low quantities of antigens.
- iPCR combines the advantages of both ELISA and PCR in specificity, sensitivity, and adaptability.
- Despite its potential, iPCR is under-utilized due to the unavailability of validated, ready-to-use commercial kits. However, we also provide our well-validated ready-to-use iPCR conversion kits and ready-to-use PicoLISA iPCR kits with our services.
- PicoLISA is an innovative assay platform that combines the specificity and simplicity of an ELISA with the sensitivity of real-time PCR, while using only 1/10th of the sample volume and providing 10X more sensitivity than ELISA.
