Axela Bio, a CRO specializing in discovery biology and Translational Sciences.
Simple Western (Automatic Western)
Advanced Western Blot Services for Targeted Protein Research: Antibody or Protein Degrader Discovery
- Relative and absolute quantitation of any protein by size from 2-440 KDa
- Quantitation of multiple target proteins / biomarkers in samples of limited amount, such as fine needle aspirates (FNA), laser capture microdissection (LCM), serum, plasma, or supernatant from live animals or human patients
- >3,000 antibodies have been validated for protein quantitation
- Real-time toxicological and PK/PD monitoring drug candidates in experimental animals
- Monitoring signaling transduction events in limited samples (as low as 25 cells), great for those from stem cells, bone marrow cells, sorted cells, etc.
- Discovery and validation of biomarkers to support developing predictive and companion diagnostics
- Characterization of monoclonal and polyclonal antibody binding affinities
- Characterization of specificity of antibody to the target protein of interest
- Characterization of purity and charge heterogeneity of biopharmaceuticals
- Phospho-protein profiling and quantitation of post-translational protein modifications using pan antibodies (after protein separation by charge/pI values) or using isoform-specific antibodies (after protein separation by size/MW)
- Screening test compounds for effects on expression, degradation or modifications of any protein or signaling molecule of interest
Traditional Western
- Our traditional Western blot services uses high-quality pre-cast gels with various chemistries (such as SDS-PAGE, Native-PAGE, etc.), as well as many well-validated specific antibodies, to obtain high resolution and consistent performance among multiple experiments.
- LiCor Odyssey Near-Infrared Imaging technology is used to scan the probed target proteins giving a wider dynamic range, two-color (green and red) detection, and quantification with higher sensitivity when compared to common chemiluminescence methods.
In-Cell Western
- Our assay combines the specificity of Western blot with the reproducibility and throughput of ELISA, which is a simple and cost-effective means for quantification of intracellular signaling in whole cells.
- This assay involves seeding cells in 96- or 384-well microtiter plates followed by fixation/permeabilization and subsequent labeling with activation state-specific or control antibodies, infrared-conjugated secondary antibodies, and/or far-red DNA dye.
- The well-level data acquired by the Odyssey® Imaging System permits accurate measurement of signal induction or inhibition, with the potential for multiple treatments, endpoints, and replicates on each plate.
- Our assays quickly and accurately measure relative protein levels in many samples, detect proteins in situ in a relevant cellular context.
- This is used for high-throughput analysis of protein phosphorylation (effects of drug compounds on signaling pathways; timing/kinetics of signal transduction), caspase-3 activation, cell surface proteins, and receptor internalization, RNAi library screens, cell proliferation, etc.
