MoA Studies

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Mechanism of Action (MoA) Studies

Understand how your drug works—before it reaches the clinic.

At Axela Biosciences, we provide a comprehensive suite of assays and platforms to uncover, confirm, and characterize the Mechanism of Action (MoA) of drug candidates. Whether you’re working with small molecules, peptides, biologics, or novel modalities like ADCs or gene therapies, we help you generate the mechanistic data needed to drive smarter decisions.

What We Can Support

Target Engagement & Binding Assays
  • Ligand-receptor binding (e.g., ELISA, FRET, SPR)
  • Enzyme inhibition/activation profiling
  • Cellular thermal shift assays (CETSA)
  • Pull-down and co-immunoprecipitation assays
Phenotypic Readouts
  • Cell proliferation, differentiation, apoptosis, and necrosis
  • Cytoskeletal reorganization, morphology changes
  • High-content imaging and live-cell analysis
  • Organelle-specific effects and subcellular localization
Omics & Multi-Modal Profiling
  • Transcriptomics and pathway mapping (800+ genes, 40+ pathways)
  • Proteomics and secretome profiling
  • Metabolomics and cellular stress response
  • Integration of genomic, proteomic, and functional data for MoA insight
Pathway & Downstream Signaling Analysis
  • Phospho-protein profiling (ELISA, Luminex, Western blot)
  • Gene expression analysis via qPCR and multiplexed RNA panels
  • Reporter gene assays
  • Transcription factor activation and nuclear translocation studies
Functional Cellular Assays
  • Cytotoxicity and target cell killing
  • Immune cell activation, cytokine release
  • Cell-cell interaction assays (e.g., effector/target co-cultures)
  • Ion channel activity and membrane potential assays
Applications Across Modalities

Our MoA studies support:

  • Small Molecules – Binding, downstream signaling, and cellular response
  • Peptides – Receptor activation, stability, and intracellular dynamics
  • ADCs & Biologics – Internalization, payload release, and cytotoxicity
  • Gene Therapy – Host-vector interactions, immune activation, gene expression
  • TPDs – Target degradation kinetics and pathway perturbation
  • Cell Therapy – Mechanistic validation of potency and specificity