Immunoassay Development

Axela offers various cost-effective immunoassays using high multiplex or ultra-sensitive platforms to obtain maximum data from miniscule samples.

Our immunoassays can be bead-based cytometric assays or chip-based antibody microarrays to quantify multiple analytes simultaneously in various samples. Samples can be serum, plasma, cell or tissue lysate, cell supernatant, milk, saliva, nasal lavage, exhaled breath condensate, urine, tear, etc.

Variety of targets or biomarkers can be quantitated (cytokines, transcription factors, protein phosphorylation, histone acetylation, mRNA, miRNA, etc.).

Our services include:

Pre-defined Panels: Choose from hundreds of validated panels, which can contain up to 80 targets or biomarkers involved in signal pathways of cancer, inflammation, autoimmunity, kidney toxicity, liver injury, metabolism, and neurodegenerative diseases among others.

Mix and Match Panels: Use the mix and match system if clients are interested in ordering a subset of specificities from one of our pre-defined panels.

Custom Panels: Design custom panels to combine analytes from multiple panels or request analytes that are not currently in our catalog. Each request will be evaluated for feasibility on a case-by-case basis.

Multiplex and Add-Mix-Read Immunoassay Development

We provide a comprehensive suite of custom immunoassay services, including assay development, validation, clinical trial testing, and CLIA-certified testing. Our expertise in antibody and antigen development helps accelerate timelines and improve assay performance.

Ensuring regulatory compliance according to client requirements
Procuring analytes and antibodies, either developed in-house or supplied by the client
Providing and validating appropriate control samples
Optimizing dilution factors for antibodies, analytes, and controls, including antibody conjugation labeling
Establishing incubation times, temperatures, and acceptance criteria through data analysis
Conducting sensitivity, precision, and reproducibility studies
Defining lower and upper limits of detection and quantitation (LLoD, ULoD, etc.)

We can seamlessly convert any ELISA assay into advanced formats such as Luminex, Lumit, AlphaLISA, DELFIA, or HTRF, streamlining workflows and reducing both time and cost.

Flow Cytometry