Luminex Multiplex Immunoassay

Luminex Multiplex Immunoassay Service

Our Luminex assays are high-throughput, multiplex bead-based assays that allow simultaneous analysis of multiple analytes (proteins and mRNA) in a single sample—using as little as 10 μL from human, mouse, or other sources. This powerful technology enables detection of:

  • ~150 mouse cytokines/chemokines

  • ~300 human cytokines/chemokines

  • Proteins involved in cancer, apoptosis, inflammation, immunity, diabetes, kidney toxicity, and more

  • Histone modifications, transcription factor activity, gene expression, and DNA copy number variation

Luminex allows:

✔️ Quantitative detection of cytokines, chemokines, cancer biomarkers, and signaling proteins in lysates, serum, plasma, and supernatants
✔️ Simultaneous multiplexing — detect proteins, peptides, or nucleic acids in a single sample volume with reproducible results
✔️ Phosphorylation & post-translational insights — interrogate multiple protein modifications in one assay
✔️ High-throughput capability — our FlexMAP 3D and Luminex-200 systems analyze hundreds of biomolecules from >10 μL samples in 96- or 384-well formats

Color-coded microspheres allow simultaneous detection of dozens to hundreds of biomarkers, delivering high-throughput, reliable results from minimal sample volume.

Applications of Luminex in Disease Biology

Immunology & Inflammation

  • Acute phase response

  • Allergy / allergen testing

  • Autoimmunity & rheumatology

  • Inflammation

  • Immune checkpoint blockade

Oncology & Cell Biology

  • Angiogenesis

  • Apoptosis

  • Cancer growth, proliferation & metastasis

  • Circulating cancer biomarkers

  • Cellular signaling

Metabolism & Chronic Diseases

  • Cardiovascular disease (CVD biomarkers)

  • Diabetes & obesity

  • Kidney injury

  • Liver injury

  • Endocrine system (pituitary, thyroid, IGFs)

  • Metabolis

`Molecular Insights

  • Protein phosphorylation

  • Gene expression profiling

  • Histone H3 PTMs

  • MicroRNA profiling

  • Transcription factor-specific DNA binding

  • Stem cell differentiation & characterization

Analytes Measured with Luminex Assays

Example Data

For more information about different Luminex assay panels available, please contact info@axelabio.com, or build customized Luminex assay using these links: ProcartaPlex, Milliplex, Bio-plex, and R&D Systems. Our scientists can also develop Luminex assays for special biomarkers.

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