Human M2a macrophages were differentiated from primary monocytes that were isolated form peripheral blood by negative immunomagnetic selection. Monocytes were differentiated for 5 days in serum-free culture media supplemented with macrophage colony-stimulating factor including a 2 day stimulation with Interleukin-4. Cells are sourced from healthy donors from world-renowned blood institutions using Institutional Review Board (IRB)-approved consent forms and protocols. Donors are screened for HIV-1, HIV-2, hepatitis B, hepatitis C, human T-lymphotropic virus, syphilis, Chagas and West Nile virus.

Source: Peripheral Blood Monocyte-Derived

Donor Status: Normal

Format: Frozen in CryoStor® CS10, shipped in dry ice

Purity: ≥ 80% CD206⁺, ≥ 80% CD209⁺, ≤ 10% CCR7⁺, and ≤ 10% CD80⁺ by flow cytometry

Viability: ≥ 95% by flow cytometry

Anticoagulant: Acid-citrate-dextrose solution A (ACDA)

Donor details: Refer to the lot-specific Certificate of Analysis (Included with product shipment)

NOTE: The certificate of analysis is mailed with all orders. An electronic version of the certificate of analysis is available upon request by emailing info@axelabio.com. Please provide the product catalog number and lot number in the request.